<p><em>Streptococcus pyogenes</em> and <em>Pseudomonas aeruginosa</em> are some of the causes of infectious diseases. <em>Centella asiatica (L.) Urban</em> has many benefits for humans, including overcoming fever, anti-bacterial, and anti-inflammatory. This study aims to determine the inhibition of <em>Centella asiatica (L.) Urban</em> leaves ethanol extract on the growth of <em>Streptococcus pyogenes</em> and <em>Pseudomonas aeruginosa</em>. This research is the initial stage of the development of herbal medicines to treat <em>Streptococcus pyogenes</em> and <em>Pseudomonas aeruginosa</em> infections. The independent variable was the concentration of ethanol extract of <em>Centella asiatica (L.) Urban</em> leaves and the dependent variable was the growth of <em>Streptococcus pyogenes</em> and <em>Pseudomonas aeruginosa</em>. The anti-bacterial activity test was carried out by the liquid dilution method. The concentrations used are 20%, 40%, 60%, 80%. 100% The results showed that the minimum inhibitory concentration (MIC) against <em>Streptococcus pyogenes</em>: 40% and <em>Pseudomonas aeruginosa</em>: 40%. Minimum bactericidal concentration (MBC) results for <em>Streptococcus pyogenes</em>: 60% and <em>Pseudomonas aeruginosa</em>: 60%. So it can be concluded that there is inhibition of the ethanol extract of <em>Centella asiatica (L.) Urban</em> leaves on the growth of <em>Streptococcus pyogenes</em> and <em>Pseudomonas aeruginosa</em>. Centella Asiatica (L.) Urban extract has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.</p>