<p><em>Citrus hystrix</em>&nbsp;D.C. is a citrus-type citrus tribal plant, one of the most favored and developed fruits by the people of South Kalimantan Indonesia.&nbsp;<em>Citrus hystrix</em>&nbsp;D.C. contains useful chemical compounds, one of which is flavonoids, which function as antibacterial and play a critical role in inhibiting bacterial growth. The purpose of this study was to determine the antibacterial activity of&nbsp;<em>Citrus hystrix</em>&nbsp;D.C. juice at 25%, 50%, 75%, and 100% concentration on the growth of&nbsp;<em>Staphylococcus aureus</em>. This research is experimental with Posttest Only Control Group Design. The material used was local&nbsp;<em>Citrus hystrix</em>&nbsp;D.C., fresh and clean green, obtained from the Astambul area, Banjar Regency, South Kalimantan Indonesia. The results showed that there was a zone of inhibition of&nbsp;<em>Citrus hystrix</em>&nbsp;D.C. juice on&nbsp;<em>Staphylococcus aureus</em>&nbsp;growth which began at 25%, 50%, 75% and 100% concentrations of 13.75mm, 17.25mm, 19.75mm, and 21.75mm, so it can conclude that the juice of&nbsp;<em>Citrus hystrix</em>&nbsp;D.C. influences the growth of&nbsp;<em>Staphylococcus aureus</em>&nbsp;which has the largest inhibitory zone at a concentration of 100%.</p>