<p>Infection is a disease caused by the presence of pathogenic microbes, including <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. Garlic (<em>Allium sativum</em> L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. This study aims to determine the antibacterial properties of garlic extract powder against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. This research is the initial stage of the development of herbal medicines to treat <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> by 54.5% and 78.5%. Garlic (<em>Allium sativum</em> L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.</p>