<p>Rosella (<em>Hibiscus sabdariffa L</em>.) is a plant that can use as herbal medicine. Roselle calyx contains active compounds flavonoids, saponins, and tannins. These compounds can use as an antibacterial against&nbsp;<em>Salmonella typhi</em>. This study aims to determine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration and the effect of rosella cooking water concentration on&nbsp;<em>Salmonella typhi</em>&nbsp;in vitro. Experimental research with posttest only controls group design through dilution method. The results of the Minimum Inhibitory Concentration (MIC) study showed clarity at concentrations of 60%, 70%, 80%, 90%, and 100%. Minimum Bactericidal Concentration (MBC) results obtained the number of colonies at a concentration of 60% by one colony, at a level of 70%, 80%, 90%, and 100% showed no colony growth. Based on the results of the study concluded that there was an influence of rosella cooking water concentration on the growth of&nbsp;<em>Salmonella typhi</em>&nbsp;in vitro.</p>