Utilization of Alternative Buffer Solutions for Staining Thin Blood smears by the Giemsa, Wright stain and Romanowsky method
DOI:
https://doi.org/10.35916/thmr.v4i1.76Abstract
To make a reliable diagnosis on haematological examination, it is necessary to examine further the morphology of the blood cells previously stained in the procedure for staining thin blood smears using a buffer solution with a standard pH of 6.4 to 6.8. One of the problems that may occur in the laboratory is that the buffer reagents are damaged, past the expiration date or running out, so alternative buffers are needed that are cheap, fast and easy to obtain. This study aims to evaluate the staining results from alternative buffers. Study used a quasi-experimental method, and using alternative buffers from bottled mineral water following SNI-01-0553 2006. The colour produced by some of these alternative buffers is almost equivalent to blood cells stained with phosphate buffer. The percentage of assessment results in the alternative buffer codes B, C, D, E and F compared with control (A) were 62.67%, 92.00%, 82.67%, 80.00% 88, 00%, and 68.00%. The use of alternative buffers for staining thin blood smears using the Giemsa, Wright stain, and Romanowsky method can be done with mineral water as an alternative buffer for sample codes C, D, E and F, while B and G cannot be used. This alternative buffer can be applied by laboratory personnel in urgent situations in limited equipment and material facilities.
References
Adewoyin AS, Nwogoh B. Peripheral blood film - a review. Ann Ib Postgrad Med. 2014 Dec; 12(2): 71-9.
Mendi I. Pengaruh konsentrasi ph buffer giemsa terhadap morfologi leukosit pada preparat sumsung tulang. Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang, 2017.
Harr RR. Resensi Ilmu Labaratorium Klinis. EGC. 2013
Hidayah AN. Perbandingan berbagai metode pengeringan preparat sebelum pengecatan terhadap morfologi sel darah merah (Erythocyte) pada apusan darah tepi. Fakultas Ilmu Kesehatan, Universitas Muhamadiyah Surabaya, 2019.
Gandasoebrata, R. Penuntun Laboratorium Klinik. Dian Rakyat. 2010
Wantini S, Huda M. Pengaruh konsentrasi dan waktu pengecatan giemsa pada pemeriksaan mikroskopik malaria. Jurnal Analis Kesehatan. 2021 Jun 28;10(1):8-13.
Hormalia, Arsyad M, Haitami. Pengaruh variasi pengenceran giemsa terhadap pewarnaan giemsa Plasmodium sp pada pemeriksaan sediaan darah tipis. Jurnal Ergasterio, 2017; 5(1)
Rahmah S, Muhlisin A, Arsyad M. Quality difference of blood smear staining with wright's method used pdam water, aquadest and buffer ph 6,8. Jurnal Ergasterio. 2018 Sep 17;5(2).
Indonesia SN. Air minum dalam kemasan. Badan Standardisasi Nasional: Jakarta. 2006.
Devirizanty D, Nurmalawati S, Hartanto C. Perbandingan unjuk kinerja berbagai tipe ph meter digital di laboratorium kimia. Jurnal Pengelolaan Laboratorium Sains dan Teknologi. 2021 Jun 15;1(1):1-9.
Wedhaswara AG. Pengaruh penundaan pembuatan preparat apusan darah tepi pada Sampel EDTA terhadap morfologi sel darah merah. Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang, 2018.
Ardina R, Rosalinda S. Morfologi eosinofil pada apusan darah tepi menggunakan pewarnaan Giemsa, Wright, dan kombinasi Wright-Giemsa. Jurnal Surya Medika (JSM). 2018 Feb 1;3(2):5-12.
Sholekha FE. Pengaruh konsentrasi larutan fiksasi terhadap hasil makroskopis dan mikroskopis sediaan apus darah tepi. Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang, 2018.
Bain BJ. Blood cells: a practical guide. John Wiley & Sons; 2021 Dec 29.
Nugraha G. Panduan Pemeriksaan Laboratorium Hematologi Dasar. CV Trans Info Medika: Jakarta. 2015.
