Differences in Thawing Methods in Broiler Chicken Meat on Total Plate Count (TPC) of Bacteria

Authors

  • Noorlaily Putri Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin, Indonesia
  • Leka Lutpiatina Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin, Indonesia
  • Nurlailah Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin, Indonesia
  • Aima Insana Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin, Indonesia

DOI:

https://doi.org/10.35916/thmr.v6i2.120

Keywords:

Broiler chicken, thawing, Total Plate Count (TPC)

Abstract

Total Plate Count (TPC) determines the number of microorganisms, both bacteria and fungi. Thawing is a step in thawing food that was previously stored frozen. This study aims to analyze the differences in the Total Plate Count of bacteria with variations in thawing methods in broiler chicken meat. Thawing methods commonly used include thawing at room temperature, soaking in water, and leaving it in the refrigerator (chiller). The type of research is experimental design with a posttest-only control design. Repetition was carried out five times for each control group KN (negative control) and test groups R0 (fresh chicken), R1 (frozen chicken), R2 (chicken thawed at room temperature), R3 (chicken soaked in water) and R4 (chicken thawed in the chiller) then the number of bacteria that grew was calculated. The average number of R0 colony results ranged from 4.1 x 103 CFU/g, R1 8.6 x 102 CFU/g, R2 1.6 x 104 CFU/g, R3 8.9 x 103 CFU/g and R4 1.2 x 103 CFU/g. The Kruskall-Wallis test results showed an Asymp sig value of 0.000 (<0.05); it can be concluded that there are differences in TPC in broiler chicken meat with variations in thawing methods that are thawed at room temperature, soaked in water and thawing in the chiller. It is recommended to thaw frozen chicken meat using the method of leaving it in the refrigerator because it has the lowest number of germs and can inhibit the growth of germs.

References

Sembor, S. M., &Tinangon, R. Industri Pengolahan Daging. Bandung: CV. Patra Media Gravindo. 2022

Mardhika, H., Dwiloka, B., & Setiani, B. E. Pengaruh berbagai metode thawing daging ayam petelur afkir beku terhadap kadar protein, protein terlarut dan kadar lemak steak ayam. Jurnal Teknologi Pangan. 2020; 4(1): 48-54.

Akhtar S, Khan MI and Faiz F. Effect of thawing on frozen meat quality: A comprehensive review. Pakistan Journal of Food Science. 2013; 23: 198-211

El Jalil MH, Khamar M, Maaninou S, Dahha M, Zinedine A and Ameur N. Antibiotic resistance of Escherichia coli strains isolated from broiler meat in Morocco. International Journal of Veterinary Science. 2020; 9: 305-30.

Sulleyman KW, Adzitey F and Boateng EF. Knowledge and practices of meat safety by meat sellers in the Accra Metropolis of Ghana. International Journal of Veterinary Science. 2018; 7: 167-171.

Diana, C., Dihansih, E., &Kardaya, D. Kualitas Fisik Dan Kimiawi Daging Sapi Beku Pada Berbagai Metode Thawing. JurnalPertanian. 2018; 2(2):130-138

Mahmoud DH, Mahmoud HB, EL-Nawawi FAM, Abdel-Naeem HHS. Impact of Thawing Methods on the Bacteriological Quality of Chicken Meat. International Journal of Veterinary Science. 2021; 10. 10.47278/journal.ijvs/2021.041.

Oliveira MR, Gubert G, Roman SS, Kempka AP and Prestes RC. Meat quality of chicken breast subjected to different thawing methods. Brazilian Journal of Poultry Science. 2015; 17: 165–172

Vera, N., Haris, M., & Wibowo, A. Efek Pencairan Daging Dengan Berbagai Metode Thawing Terhadap Karakteristik Kualitas Daging Sapi Beku. Jurnal Peternakan Lingkungan Tropis. 2021; 4(1).

Zhang X, Gao T, Song L, Zhang L, Jiang Y, Li J, Gao F and Zhou G. Effects of different thawing methods on the quality of chicken breast. International Journal of Food Science and Technology. 2017; 52: 2097–2105

Ratminingsih, N. M. Penelitian eksperimental dalam pembelajaran bahasa kedua. Prasi: Jurnal Bahasa, Seni, Dan Pengajarannya. 2010; 6(11).

Edi, S., & Rahmah, R. S. N. Pengaruh Lama Penyimpanan Daging Ayam Pada Suhu Ruang dan Refrigerator Terhadap Angka Lempeng Total Bakteri Dan Adanya Bakteri Salmonella Sp. JBIO. Jurnal Biosains (The Journal Of Biosciences). 2018; 4(1): 23-31.

Patantis, G., Chasanah, E., Zilda, D. S., & Waluyo, I. B. Bacterial Diversity Of The Deep Sea Of Sangihe Talaud, Sulawesi. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology. 2012; 7(1), 19-27.

Rosalina, Anica G. Kajian Energi Pembekuan Daging Sapi Menggunakan Mesin Pembeku Tipe Lempeng Sentuh Dengan Suhu Pembekuan Berubah. Skripsi. Departemen Teknik Pertanian Fakultas Teknologi Pertanian, Institut Pertanian Bogor. 2010.

Firdausyi, A. A., Estoepangestie, A. T. S., Wibawati, P. A., Hamid, I. S., Solikhah, T. I., &Prastiya, R. A. Angka Lempeng Total Daging Ayam Broiler di Beberapa Pasar di Kota Bekasi pada Maret 2021. Jurnal Medik Veteriner. 2022; 5(2), 157-161.

Irmayani, Rasbawati, I. D. Novieta, & Nurliyani. AnalisisCemaranMikroba dan Nilai pH Daging Ayam Broiler di Pasar TradisionalLakessi Kota Pare-Pare. Jurnal Galung Tropika. 2019; 8(1): 1-8. http://dx.doi.org/10.31850/jgt.v8i1.431.

Belletti N, Kamdem SS, Patrignani F, Lanciotti R, Covelli A and Gardin F. Antimicrobial activity of aroma compounds against Saccharomyces cerevisiae and improvement of microbiological stability of soft drinks as assessed by logistic regression. Applied and Environmental Microbiology. 2007; 73: 5580–5586

Erdem, A. K., Saglam, D., Ozer, D., & Ozcel?k, E. Microbiological quality of minced meat samples marketed in Istanbul. Van Veterinary Journal. 2014; 25(3), 67-70.

Xia X, Kong B, Liu J, Diao X and Liu Q. Influence of different thawing methods on physicochemical changes and protein oxidation of porcine longissimus muscle. LWTFood Science and Technology. 2012; 46: 280-286.

He X, Liu R, Nirasawa S, Zheng D and Liu H. Effect of high voltage electrostatic field treatment on thawing characteristics and post-thawing quality of frozen pork tenderloin meat. Journal of Food Engineering. 2013; 115: 245-250.

Leygonie C, Britz TJ and Hoffman LC. Impact of freezing and thawing on the quality of meat. Meat Science. 2012; 91: 93-98.

Lawrie. Ilmu daging. (Penerjemah A. Parakkasi dan Yudha A). Universitas Indonesia Press, Jakarta. 2003.

Afshari, A., A. Baratpour, S. Khanzade, & A. Jamshidi. Salmonella enteritidis and Salmonella typhimorium Identification in Poultry Carcasses. Iran J. Microbiol. 2018; 10(1): 45-50.

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Published

31-08-2024

How to Cite

Putri, N., Lutpiatina, L., Nurlailah, & Insana, A. (2024). Differences in Thawing Methods in Broiler Chicken Meat on Total Plate Count (TPC) of Bacteria. Tropical Health and Medical Research, 6(2), 21–28. https://doi.org/10.35916/thmr.v6i2.120