Antibacterial Effectiveness of A Combination of Anredera cordifolia (Ten.) Steenis and Strobilanthes crispus Blume Extract on Inhibition of the Growth of Streptococcus sp. Causes of Diabetic Ulcers

Ulfiyah Khairani; Tiara Dini  Harlita; Ganea Qorry  Aina

doi:10.35916/thmr.v6i2.117

Authors

Ulfiyah Khairani Health Polytechnic of the Ministry of Health East Kalimantan
Tiara Dini Harlita Health Polytechnic of the Ministry of Health East Kalimantan
Ganea Qorry Aina Health Polytechnic of the Ministry of Health East Kalimantan

DOI:

https://doi.org/10.35916/thmr.v6i2.117

Keywords:

Antibacterial effectiveness, ceftriaxone, diabetic ulcers, Streptococcus sp

Abstract

Diabetic ulcers are open wounds on the skin surface due to macroangiopathy complications that become infected due to the entry of bacteria. Binahong and keji beling plants, can be used to treat diseases known as antibacterial and anti-inflammatory. This study aimed to determine the antibacterial effectiveness of a combination of binahong (Anredera cordifolia Ten. Steenis) and keji beling (Strobilanthes crispus Blume) extracts against Streptococcus sp. bacteria that cause diabetic ulcers. Samples were extracted using 96% ethanol solvent and then diluted the extract with a concentration variation of 25%, 50%, 75%, and 100%—antibacterial test using disc diffusion method (Kirby Bauer). Data analysis was used for the One-way ANOVA test and Duncan's Multiple Range Test further test method, which had a significance level of 0.05. Determination of antibacterial effectiveness was done by comparing the diameter of the most effective inhibition zone with the control antibiotic Ceftriaxone 30 µg. The results showed that the mean diameter of the inhibition zone of 25% concentration was 7.10 mm, and the inhibition zone of 100% concentration was 9.20 mm with moderate inhibition response. Statistical One Way Anova test showed that the combination of extracts affected the growth of Streptococcus sp. The most effective Concentration is at 100% concentration of 9.20 mm and antibacterial effectiveness of 92% with positive control Ceftriaxone 30 µg at 10.00 mm. It can be concluded that the combination of binahong and keji beling extracts can inhibit Streptococcus sp. bacteria, but its potential is not yet effective compared to Ceftriaxone 30 µg antibiotics

References

Djamil R, Kartika Pratami D, Vidia Riyantika L. Quality Parameters Test and ?-Glukosidase Enzyme Inhibitory Activity of 70% Ethanol Extract Keji Beling Leaves (Sericocalyx Crispus (L.) Bremek). Jornal of Herbal Medicine. 2020;5(1):1–8.

Aryzki S, Alicia M, Rahmah S. Overview of Antibiotic Use in Diabetic Ulcer Patients in the Internal Medicine Outpatient Installation of Rsud Ulin Banjarmasin Period July - December 2018. Manuntung Scietific Journal. 2020;6(2):265–72.

Anwar TM, Soleha TU. Benefits of Binahong Leaf (Anredera cordifolia) as a therapy for Acne Vulgaris. Majority. 2016;5(5):179–83.

Halim HA, Ratnah S, Abdullah T. Phytochemical Screening and Potential Antibacterial Activity of Binahong (Anredera Cordifolia (Ten.) Steenis) Leaf Extract against Staphylococcus aureus and Escherichia coli. Journal of Labora Medika 2022;6(2):49–52. http://jurnal.unimus.ac.id/index.php/JLabMed

Wisudawan, Arsal ASF, Imron A, Bamahry A, Makmun A. Test of Inhibitory Effectiveness of Binahong Leaf Extract against the Growth of Candida albicans. Fakumi Med J. 2021;3(5):254–60.

Rani A, Haris NM, Faisal TA. Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis) Against Blood Sugar Levels of Mice. In: Proceedings of the National Seminar on Research Results. 2020. p. 484–90:

Ainurrochmah A, Ratnasari E, Lisdiana L. Effectiveness of Binahong Leaf Extract (Anredera cordifolia) on Growth Inhibition of Shigella flexneri Bacteria by the Well Method. Journal of LenteraBio. 2013;2(3):233–7.

Darsana, I.G., Besung, I.N., & Mahatmi, H. The potential of Binahong Leaf (Anredera cordifolia (Tenore) Steenis) in Inhibiting the Growth of Escherichia coli Bacteria In Vitro. Indones Med Veterinus. 2012;1(3):337–51.

Mengga C, Rampe M, Sangande F. Antibacterial Effectiveness Test of Binahong Leaf Extract (Anredera cordifolia (Tenore) Steenis) Against Staphylococcus aureus Bacteria. Biofarmasetikal Trop. 2022;5(1):60–5.

Sasebohe VY, Prakasita VC, Aditiyarini D. Antibacterial Activity of Binahong Leaf Ethanol Extract Against Staphylococcus aureus and Propionibacterium acnes that Cause Acne. Sciscitatio. 2023;4(1):1–10.

Roring N, Yudistira A, Lolo WA. Standardization of Specific Parameters and Anticancer Activity Test against T47D Breast Cancer Cells from Ethanol Extract of Keji Beling Leaf (Strobilanthes Crispa (L.) Blume). Pharmacon. 2017;6(3):176–85.

Rawung I, Wowor PM, Mambo C. Inhibition Test of Keji Beling Leaf Extract (Sericocalyx crispus (L). Bremek) against the Growth of Streptococcus pyogenes. J e-Biomedik. 2019;7(2):125–9.

Dita Artanti SF. Effectiveness of Keji Beling (Sericocalyx crispus Linn) Leaf Juice in Inhibiting the Growth of Staphylococcus aureus. J Muhammadiyah Med Lab Technol. 2017;1(1):78–83.

Benigna M. Inhibition Test of Keji Beling Leaf Extract (Srobilanthes crispa Bl.) Against the Growth of Salmonella typhi Bacteria In Vitro. Universitas Sanata Dharma; 2015.

Adibi S, Nordan H, Ningsih SN, Kurnia M, Evando, Rohiat S. Antioxidant and Antibacteria Activities Of Strobilanthes crispus Bl (Keji Beling) Extract Againts Stapylococcus aureus and Escherichia coli. ALOTROP Jornal of Chemical Education and Science. 2017;1(2):148–54.

Helianto RL. Antibacterial Activity Test of Keji Beling (Strobilanthes crispus) Leaf Extract against Streptococcus mutans Bacteria. 2022: https://repositori.usu.ac.id/handle/123456789/50291

Charismawati NA. Antibacterial Effectiveness Test of Combination of Binahong Leaf Extract (Basella cordifolia Lam.) and green betel leaf extract (Piper betel L.) against Escherichia coli bacteria by diffusion method. Stikes Bhakti Husada Mulia Madiun; 2019.

Fitriana A, Harun N, Yusmarini Y. The Quality of Herbal Tea Leaves Keji Beling With Drying Time Treatment. J Sagu. 2017;16(2):34–41.

Samirana PO, Swastini DA, Ardinata IPR, Suarka IPSD. Determination of Chemical Content Profile of Binahong Leaf Ethanol Extract (Anredera scandens (L.) Moq.). Journal of Udayana Farmasi. 2017;6(1):23–5.

Afrida Amaliah LL. Antibacterial Activity of Ethanol Extract Combination of Binahong and Basil Leaves to The Growth of Escherichia coli. Journal of Lentera Bio. 2022;11(3):603–10: https://journal.unesa.ac.id/index.php/lenterabio/index603

Pancawati Ariami, Igan Danuyanti BRA. Effectiveness of Mangosteen Fruit Peel Tea (Garcinia mangostana L) as an Antimicrobial Against the Growth of Methicillin-Resistant Staphylococcus aureus (MRSA) Bacteria. Journal of Laboratory Technology. 2017;3(6):3–8.

Harlita TD, Anggrieni N, Rahmawati AAW. Antibacterial Activity and Effectiveness of Ciplukan Leaf Extract (Physalis angulata L.) Against the Growth of Bacillus cereus. Husada Mahakan Health Journal. 2019;V(1):51–60.

Harlita TD, Oedjijono, Asnani A. The Atibacterial Activity of Dayak Onion (Eleutherine palmifolia (L.) merr) Towards Pathogenic Bacteria. Trop Life Sci Res. 2018;29(2):39–52.

Usman I, Rambung JS, Hijriah ER, Ismail. Antibacterial Activity Of Extracts Of Basil Leaves And Binahong Leaves On Streptococcus mutans. Journal of Pharmaceutical Media. 2019;15(2):107–11.

Tilarso DP, Muadifah A, Handaru W, Pratiwi PI, Khusna ML. Antibacterial Activity of Combination of Betel Leaf and Belimbing Wuluh Extracts by Hydroextraction Method. Chempublish J. 2021;6(2):63–74.

Herrialfian, Lubis MMN, Darmawi, Dewi M, Erina, Hennivanda AH. Inhibition Activity of Ethanolic Extract of Binahong Leaf (Anredera cordifolia (Ten.) Steenis) on Staphylococcus aureus Bacteria. Journal of Medica Veterinaria. 2022;15(1):43–55.

Setyoningsih H, Yudanto GP, Ismah K, Handayani Y. Evaluation of Antibiotic Use in Diabetes Patients Based on the Gyssens Method at Kudus Islamic Hospital. Cendikia J Pharm. 2015;6(2):257–69.