Number of Breast Milk Bacteria in Women with Normal Labor and Caesarean Delivery During Room Temperature Storage

Authors

  • Siti Noor Habibah Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin
  • Ahmad Muhlisin Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin
  • Leka Lutpiatina Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin
  • Aima Insana Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin

DOI:

https://doi.org/10.35916/thmr.v6i1.111

Keywords:

Bacteria count, breast milk, cesarean delivery, normal birth

Abstract

The presence of microbes in each breast milk sample can be different, one of which is influenced by the method of delivery. The research aimed to analyze the effect of storage time for breast milk from mothers giving birth normally and giving birth by Caesarean on the number of bacteria at room temperature. The independent variable in this study was the storage time for breast milk from mothers giving birth normally and giving birth by Caesarean for 0, 4, 8, and 12 hours at room temperature, while the dependent variable in this study was the number of bacteria in the breast milk. The research samples were breast milk from mothers who gave birth normally and Caesarean section which was treated with storage at room temperature for 0, 4, 8, and 12 hours. The results of the research were that the average number of bacteria in breast milk from mothers giving birth normally, stored at 0, 4, 6, 8, and 12 hours respectively, was 1.33 x 104 CFU/ml; 2.18 x 104 CFU/ml; 5.04 x 106 CFU/ml; 1.68 x 107 CFU/ml. The average number of bacteria in breast milk from mothers giving birth by Caesarean storage 0, 4, 6, 8, and 12 hours respectively 1.83 x 104 CFU/ml; 1.73 x 105 CFU/ml; 1.76 x 107 CFU/ml; 6.67 x 107 CFU/ml. There is a significant difference in the number of bacteria between breast milk from mothers who gave birth normally and breast milk from mothers who gave birth by Caesarean section. Storage time has a positive effect on the number of bacteria in breast milk from mothers giving birth normally and giving birth by Caesarean. It is recommended to store breast milk at room temperature for up to 4 hours for breast milk from women giving birth normally and for breast milk from mothers giving birth by Caesarean, storage for less than 4 hours by paying attention to the cleanliness of the breast milk storage area.

References

Martín, R., Langa, S., Reviriego, C., Jiménez, E., Marín, M. L., Xaus, J., Fernández, L., & Rodríguez, J. M. Human Milk Is A Source of Lactic Acid Bacteria For The Infant Gut. Journal of Pediatrics. 2003; 143(6):754–758

Perez PF, Doré J, Leclerc M, Levenez F, Benyacoub J, Serrant P, Segura-Roggero I, Schiffrin EJ, Donnet-Hughes A. Bacterial imprinting of the neonatal immune system: lessons from maternal cells? Pediatrics. 2007 Mar; 119(3):e724-32. doi: 10.1542/peds.2006-1649

Werdayanti, R. Bapak ASI dan Ibu Bekerja Menyusui. Familia: Jakarta, 2015

Siswosuharjo, S., & Chakrawati, F. Panduan Super Lengkap Hamil Sehat (1st ed.). Penebar Plus: Jakarta, 2010

Viandika N and Septiasari RM. Pengaruh continuity of care terhadap angka kejadian Sectio Cessarea. J. Qual. Women’s Heal. 2020; 3(1):1-8 doi: 10.30994/jqwh.v3i1.41

Li, S. W., Watanabe, K., Hsu, C. C., Chao, S. H., Yang, Z. H., Lin, Y. J., Chen, C. C., Cao, Y. M., Huang, H. C., Chang, C. H., & Tsai, Y. C. Bacterial Composition And Diversity In Breast Milk Samples From Mothers Living In Taiwan And Mainland China. Frontiers in Microbiology. 2017; 8(MAY):1–15.

Eglash, A., & Simon, L. ABM Protocol. Breastfeeding Medicine. 2017; 12(7): 1– 6.

Siahaya, G. C., & Talarima, B. Pengaruh Lama Penyimpanan Air Susu Ibu (ASI) Pada Suhu -15oC Terhadap Kualitas ASI. 2-Trik: Tunas-Tunas Riset Kesehatan. 2017; 7(2): 23-33

Mustika, D. N., Nurjanah, S., Noor, Y., Ulvie, S., & Semarang, U. M. Identifikasi Total Bakteri dan Keasaman Air Susu Ibu Perah (ASIP) Yang Disimpan Di Cooler Bag. Jurnal Gizi. 2019; 8(36):28–36.

Arslanoglu, S., Bertino, E., Tonetto, P., Nisi, G. D. E., Ambruzzi, A. M., Biasini, A., Profeti, C., Spreghini, M. R., Association, I., Banks, M., Italiana, A., Umano, L., Aiblud, D., Intensive, N., Unit, C., Intensive, N., Unit, C., Hospital, S. C., Melloni, M., & Hospital, M. Guidelines For The Establishment And Operation of A Donor Human Milk Bank. The Journal of Maternal-Fetal and Neonatal Medicine, 2010; 23(September):1–20.

Mutaqin, L., Dlh, D., Husin, F., Melinda, H., Nataprawira, N., Kesehatan, D., Bima, K., Tenggara Barat, N., Ilmu, D., Anak, K., Kunci, K., Penyimpanan, D., & Asi, K. The Effect of The Duration of Storage of Room Association On Quality of Breastmilk. Jurnal Kesehatan Prima. 2018; 13(1):51–59.

Cabrera-Rubio, Raul, Collado, M. C., Laitinen, K., Salminen, S., Isolauri, E., & Mira, A. The Human Milk Microbiome Changes Over Lactation And Is Shaped By Maternal Weight And Mode Of Delivery. American Journal of Clinical Nutrition. 2012; 96(3):544–551.

Cabrera-Rubio, R., Mira-Pascual, L., Mira, A., & Collado, M. C. 2016. Impact of Mode of Delivery On The Milk Microbiota Composition of Healthy Women. Journal of Developmental Origins of Health and Disease. 2016; 7(1):54–60.

CDC. Storage And Preparation Of Breast Milk. 1–2. 2019. https://www.cdc.gov/breastfeeding/recommendations/handling_breastmilk.htm, accessed on 29-02-2024

Murwani, S. Dasar-Dasar Mikrobiologi Veteriner. Universitas Brawijaya Press: Malang, 2015

Pranatami, D. A. Perbandingan Jumlah Total Bakteri pada Penggunaan Wadah Penyimpanan Air Susu Ibu ( ASI ) yang Berbeda. Al-Hayat: Journal of Biology and Applied Biology. 2020; 3(1):15–20.

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Published

31-03-2024

How to Cite

Habibah, S. N., Muhlisin, A., Lutpiatina, L., & Insana, A. (2024). Number of Breast Milk Bacteria in Women with Normal Labor and Caesarean Delivery During Room Temperature Storage. Tropical Health and Medical Research, 6(1), 10–19. https://doi.org/10.35916/thmr.v6i1.111

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