Tropical Health and Medical Research

Healing Effect of Wistar Rat Incision Wounds on Administration of Microemulsion Combination of Green and Red Betel Leaf Extracts

2023-11-24T08:18:36+07:00

One natural ingredient that has the potential to heal wounds is betel leaf. This study aimed to analyze the healing effect of Incision wounds in Wistar rats when administering a microemulsion combination of green and red betel leaf extracts. This research begins with sample collection, drying process, and extraction. The extract was then formulated into emulgel as a microemulsion in three formulations. These three formulas, along with a positive control and a negative control, were tested on each Wistar rat incision wound. The results of the research showed that emulgel formula 3, which was tested on mice, had the best ability to heal wounds for an average of 2.5 days and kept the mice's body temperature normal; 36.6^oC. Formula 3 contains 5% South Kalimantan red betel, 5% South Kalimantan green betel, 5% Central Kalimantan red betel, and 5% Central Kalimantan green betel. This proves that the combination of formula 3 can heal wounds and body temperature in experimental mice. Further research is needed to find the best composition that produces faster, more complete wound healing effects and safely impacts experimental animals.

Number of Breast Milk Bacteria in Women with Normal Labor and Caesarean Delivery During Room Temperature Storage

2024-03-30T06:42:27+07:00

The presence of microbes in each breast milk sample can be different, one of which is influenced by the method of delivery. The research aimed to analyze the effect of storage time for breast milk from mothers giving birth normally and giving birth by Caesarean on the number of bacteria at room temperature. The independent variable in this study was the storage time for breast milk from mothers giving birth normally and giving birth by Caesarean for 0, 4, 8, and 12 hours at room temperature, while the dependent variable in this study was the number of bacteria in the breast milk. The research samples were breast milk from mothers who gave birth normally and Caesarean section which was treated with storage at room temperature for 0, 4, 8, and 12 hours. The results of the research were that the average number of bacteria in breast milk from mothers giving birth normally, stored at 0, 4, 6, 8, and 12 hours respectively, was 1.33 x 10⁴ CFU/ml; 2.18 x 10⁴ CFU/ml; 5.04 x 10⁶ CFU/ml; 1.68 x 10⁷ CFU/ml. The average number of bacteria in breast milk from mothers giving birth by Caesarean storage 0, 4, 6, 8, and 12 hours respectively 1.83 x 10⁴ CFU/ml; 1.73 x 10⁵ CFU/ml; 1.76 x 10⁷ CFU/ml; 6.67 x 10⁷ CFU/ml. There is a significant difference in the number of bacteria between breast milk from mothers who gave birth normally and breast milk from mothers who gave birth by Caesarean section. Storage time has a positive effect on the number of bacteria in breast milk from mothers giving birth normally and giving birth by Caesarean. It is recommended to store breast milk at room temperature for up to 4 hours for breast milk from women giving birth normally and for breast milk from mothers giving birth by Caesarean, storage for less than 4 hours by paying attention to the cleanliness of the breast milk storage area.

Decreasing the Number of Leukocytes as an Effect of Standard Therapy and Rhodomyrtus tomentosa Leaves Extract Therapy

2024-04-02T07:48:45+07:00

The postpartum period is a vulnerable period to infection due to low immunity. An increase in leukocytes characterizes the body's response to infection; several leukocytes close to normal values indicate that the infection period will end. The research objective was to determine the effectiveness of Rhodomyrtus tomentosa leaf extract on the number of leukocyte cells in mothers with postpartum infections at Tarakan City General Hospital. The research used an Analytical survey with a sample of postpartum mothers who came to the Tarakan City General Hospital (April-August 2022). Criteria for Group 1 were mothers with postpartum infections and had not been given any therapy; Group 2 were healthy postpartum mothers; Group 3 were mothers with postpartum infections who were given standard therapy; Group 4 is mothers with postpartum infections who were given standard therapy and on the doctor's advice the day before they were given Karamunting leaf extract as additional therapy. The research results show that giving Rhodomyrtus tomentosa leaf extract with standard therapy reduces the number of leukocytes to close to normal values, so it can be concluded that it has an anti-inflammatory effect on mothers with postpartum infections. Further clinical trial research is needed with adequate samples and more uniform conditions for each sample.

Validation of Questionnaires and the Effect of Educational Videos on the Knowledge of Hyperlipidemia Patients at Banjarbaru Utara Primary Health Care

2024-04-08T08:13:11+07:00

Hyperlipidemia patients have a high risk of cardiovascular disease and stroke. Low patient knowledge causes therapy failure. Education can be provided to overcome this problem. No questionnaire instrument can measure the level of knowledge of hyperlipidemia patients. The level of knowledge of hyperlipidemia patients at Banjarbaru Utara Primary Health Care has never been studied. The research aimed to validate the questionnaire and determine the effect of educational videos on patient knowledge at the Banjarbaru Utara Primary Health Care, Banjarbaru City, South Kalimantan. The research method used to validate the questionnaire was cross-sectional, while to determine the effect of educational videos on the patient's level of knowledge using a quasi-experimental method using One Group Pre – Post Design. The research was conducted in January – March 2024 at Banjarbaru Utara Primary Health Care. The study involved eight experts, 40 patients in questionnaire validation, and 100 patients to determine the effect of educational videos. The results of questionnaire content validation show that the Content Validity Ratio (CVR) value is in the range of 0.750 – 1.000, while the Content Validity Index (CVI) value is in the range of 0.875 – 1.000. The validity test with Person Correlation shows that ten out of 20 statements on the questionnaire are considered valid (>0.312). The Cronbach's Alpha value is 0.655, indicating the questionnaire is reliable. The patient's knowledge before being given the educational video had a score of 5.96 (scale 1 – 10), while after it, it had a score of 8.73. There was an increase in the level of knowledge, reaching 46.47%. The conclusion is that the Hyperlipidemia Knowledge Questionnaire (HKQ), which consists of 10 statements, is declared valid and reliable. Educational videos significantly influence (p-value 0.000) in increasing knowledge of hyperlipidemia patients.

Salmonella sp Contamination on Chicken Eggs and Duck Eggs at the Antasari Traditional Market, Banjarmasin, Indonesia

2024-04-16T09:08:09+07:00

Chicken eggs and duck eggs can be a source of transmission of Salmonellosis. Salmonella bacteria can penetrate the eggshell, or the egg's contents can become directly contaminated with Salmonella in the reproductive organs before the shell covers the egg. This research aims to analyze Salmonella sp in chicken and duck eggs at the Antasari Traditional Market in Banjarmasin, Indonesia. Samples were taken by purposive sampling with the criteria for cracked/broken eggs and intact eggs: 3 chicken eggs and three duck eggs from 6 traders so that the total sample was 24. For the identification of bacteria, microscopic tests and biochemical tests were used on the identification media. The results showed Salmonella sp in duck eggs and no Salmonella in chicken eggs. In all samples of chicken and duck eggs, 12.50% of Salmonella sp, 37.50% of Klebsiella sp, 12.50% of Proteus sp, and 37.50% of Pseudomonas sp were found. Suggestions for consumers are to pay more attention to the hygiene and sanitation of traders before buying eggs, choose clean eggs, store eggs in the refrigerator, and cook eggs until they are cooked.